|
KMID : 1036720140470010012
|
|
Journal of Nutrition and Health
2014 Volume.47 No. 1 p.12 ~ p.22
|
|
|
Exploration of optimum conditions for production of saccharogenic mixed grain beverages and assessment of anti-diabetic activity
|
|
Lee Jae-Sung
Kang Yun-Hwan
Kim Kyoung-Kon
Yun Yeong-Kyeong
Lim Jun-Gu
Kim Tae-Woo
Kim Dae-Jung
Won Sang-Yeon
Bae Moo-Hoan
Choi Han-Seok
Choe Myeon
|
|
|
Abstract
|
|
|
Purpose: This study was conducted to establish the production conditions through optimization of the production process of beverages using Aspergillus oryzae CF1001, and to analyze volatile compounds and antidiabetic activity.
Methods: The optimum condition was selected using the response surface methodology (RSM), through a regression analysis with the following independent variables gelatinization temperature (GT, X1), saccharogenic time (ST, X2), and dependent vari-able; ΔE value (y). The condition with the lowest ΔE value occurred with combined 45 min ST and 50℃ GT. The volatile compounds were analyzed quantitatively by GC-MS.
Results: Assessment of antidiabetic activity of saccharogenic mixed grain beverage (SMGB) was determined by measurement of α-glucosidase inhibition activity, and glucose uptake activity and glucose metabolic protein expression by reverse transcriptase polymerase chain reaction (RT-PCR) and western blot analysis. Results of volatile compounds analysis, 62 kinds of volatile compounds were detected in SMGB. Palmitic acid (9.534% ratio), benzaldehyde (8.948% ratio), benzyl ethyl ether (8.792% ratio), ethyl alcohol (8.35% ratio), and 2-amyl furan (4.826% ratio) were abundant in SMGB. We confirmed that α-glucosidase inhibition activity, glucose uptake activity, and glucose-metabolic proteins were upregulated by SMGB treatment with concentration dependent manner.
Conclusion: Saccharogenic mixed grain beverage (SMGB) showed potential antidiabetic activity. Further stud-ies will be needed in order to improve the taste and functionality of SMGB.
|
|
|
KEYWORD
|
|
|
response surface methodology, volatile compounds, saccharogenic mixed grain beverage, α-glucosidase, anti-diabetic activity
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|